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Histopathological analyses and immunohistochemistry Animals were sacrificed with carbon dioxide and perfused with PBS followed by 4% formaldehyde and post-fixed in formaldehyde for 24 h followed by 3 d in 40% sucrose, snap frozen in iso-pentane and kept at 80 C

Rockefeller has an incredibly rich history of research on the endoplasmic reticulum, so we know that when things go wrong in this organelle, many diseases ranging from neurodegeneration to cancer can result, says Birsoy