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Cell transfection pSLenti-GADD45-3xFlag (GADD45), control pSLenti-MCS-3xFlag (vector), pLenti-U6-siGADD45-CMV (siGADD45:GCATACTCCTTCCACGTTA), and control pSLenti-U6-shNC2-CMV (siNC: TTCTCCGAACGTGTCACGT) were constructed by OBiO Technology (Shanghai, China)

Management approaches include increasing fiber intake gradually (too much fiber at once worsens bloating), drinking at least 64 ounces of water daily, taking magnesium citrate (400-600mg at bedtime), and increasing physical activity