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Supplementary material The Supplementary Material for this article can be found online at: Supplementary Figure 1 Chest CT image showing a 0.3 cm nodule (red arrow) in the left lower lobe, bronchiectasis in the lower lobes bilaterally, minimal bilateral chronic inflammatory changes, and slight aortic wall calcification

In a Phase I single-blind, placebo-controlled trial, 3 male volunteers received subcutaneous MT-2 injections (0.01-0.03 mg/kg) on alternating days for 2 weeks

Taken together, these data suggest the CTD is inhibitory to PLC activity, and this inhibition is through direct binding of the distal CTD to the catalytic core of PLC2 CTD movement is important for activation of PLC To determine if the movement of the distal CTD upon G binding observed by HDX-MS is important for activation we generated a construct lacking the flexible linker between the proximal CTD and distal CTD (PLC2--linker) (Figure 6A)

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