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After transfection, the HEK293T cells were detached from plate wells by trituration with 5mL of Opti-MEM I Reduced Serum Medium (Gibco TM , Cat.# 31985070) per well

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Tax1-binding protein 1 (TAX1BP1) serves as an autophagic receptor, orchestrating the degradation of GPX4, which collectively leads to ferroptosis in response to copper stress