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More research is required in this area since manipulation of the GR regulatory system is currently an intriguing target for the treatment of stress-related illnesses (168)

and Research, Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks, FDA , Apr

The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content

To ultimately control these variables and provide traceability across differing LCMS platforms and enrichment antibodies, a whole protein standard is required