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Then the tissue sections were transferred onto Millicell cell culture inserts (0.4 M, 30 mm diameter) and placed in 6-well plates containing 1.5 mL of medium consisting of DMEM/F12 with N-2 Supplement and 1% antimycotic/antibiotic

Serum and gastric levels of the pro-inflammatory cytokines TNF- (BT-Lab E0764Ra), IL- (BT-Lab E0119Ra) and IL-10 (BT-Lab E0108Ra) were measured by using rat ELISA kits according to the manufacturer's procedure