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Sections were washed 3 times with 0.05% Triton X-100 in PBS and incubated with secondary antibodies (Molecular Probes or Thermo Fisher) and DAPI (Sigma-Aldrich, D9542) during 1 h at room temperature

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Cleavage of either FITC or MPA from the AuNPmolecular beacons in the presence of matriptase exhibited a quantifiable fluorescence signal

The study found that early fasting could reduce EAE severity by improving spinal cord demyelination, inhibiting IFN- and TNF- secretion, and increasing IL-10 production in spleen cells