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Sci Rep 16 , 5974 (2026)

The analyses of 4MSOB ITC and its conjugates were conducted using an Agilent HP1260 Series instrument coupled to an API5000 tandem mass spectrometer (AB Sciex, Darmstadt, Germany) by loading extracted samples onto an Agilent Zorbax Eclipse XDB-C18 column (50 4.6 mm 1.8 m, Agilent Technologies, Waldbronn, Germany) with mobile phase A (0.05% formic acid in milliQ water) and mobile phase B (acetonitrile)

Other limitation of our study was that there was only one trial for eicosapentaenoic acid, folic acid, lithium, and silymarin

The N-terminus and His-13 are crucial for Cu 2+ binding, and His-6 and His-14 are also implicated