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10.3390/ijms22158338 79 JavitchJ

Redox Biol 14:618625

We then adapted an all-in-one CRISPRi vector co-expressing green fluorescent protein (GFP) 48 by inserting a capture sequence in the single guide RNA (sgRNA) scaffold, enabling direct capture of sgRNAs during scRNA-seq using a 10x Genomics platform 49 , thereby supporting screening in primary cell culture systems 50