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Once Cu is in bile it forms an unabsorbable complex, possibly with certain bile acids, which is eliminated in stool ( Figure 1 ) (30)

Each experiment was made with samples from at least three donors (Table 1)

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Ascorbate can be regenerated from its oxidized forms, monodehydroascorbate (MDHA) and dehydroascorbate (DHA), by MDHA and DHA reductases (MDHARs and DHARs), respectively (Smirnoff, 2018) The activity of DHARs is coupled to oxidation of glutathione, which is also required for spontaneous, non-enzymatic reduction of DHA to ascorbate (Figure 1)