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These results clearly suggest that the treatment with MMP-3 stimulates the expression of Rac1b, which increases intracellular ROS, leading to the induction of EMT, suggesting that MMP-3 inhibitor or the inhibitors of ROS could be useful in the reversal of EMT or the killing of EMT-type cells or cancer stem cells leading to decreased tumor aggressiveness (Figure 4)

Sartorius) on his-tagged, biotinylated peptide captured on streptavidin beads (PolyAN) and incubated for 90 min with hybridoma supernatant and Atto 488-coupled isotype-specific monoclonal mouse-anti-rat IgG secondary antibodies

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