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Frozen human primary monocytes were obtained from Penn Human Immunology Core, then thawed and cultured in RPMI1640 with 10% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), and 10 U mL 1 penicillin-streptomycin (Gibco)

doi: 10.1158/2159-8290.CD-19-0761 106 MotwaniMPesiridisSFitzgeraldKA

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The Precursor Approach: NAC and the Rise of GlyNAC Because getting glutathione into cells directly is difficult, a parallel strategy focuses on supplying what cells need to make it themselves