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Briefly, after treatment with carboplatin, cells were incubated with DCFH-DA at 37 C, and ROS-mediated oxidation of the fluorescent compound DCF was measured using a Reactive Oxygen Species Assay Kit (S0033s, Beyotime Biotechnology) according to the manufacturers instructions

& Rakoff-Nahoum, S

Ferroptosis and pyroptosis represent two distinct modes of regulated necrotic cell death, both of which have gained recognition in recent years