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Even though alternative models are available, such as animal models or ex vivo models, in vitro models offer higher experimental flexibility and reproducibility, making them a valuable tool for the understanding of pathophysiological mechanisms of several diseases, such as CVDs

PMID 34776024

Moreover, the CG hypomethylation in neurons frequently co-occurs with human neuron-specific histone H3-trimethyl-lysine 4 (H3K4me3) modifications, indicating that CG hypomethylation facilitates an active chromatin landscape in the prefrontal cortex in a cell-type-specific manner

Two-way analysis of variance (ANOVA) followed by Tukeys Post-Hoc test was performed using GraphPad Prism 9 for multiple comparisons among marker metabolites and metabolite classes at a significance level less than 0.05