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Description

For SDS-PAGE, the proteins in the Affi-eluates (1020 L) were reduced by -mercaptoethanol or sodium dithionite (DTT, 100 mM), then separated by SDS-PAGE (8% gels), and transferred to PVDF membranes (0.2 micron)

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In fact, this method may be useful during drug discovery stages to generate hypotheses and mitigate bioactivation mechanisms

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