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Blood samples were collected from dogs administered multiple doses at corresponding time points before the first dosing (0 h), within 6 h after dosing, before the last three doses, and at corresponding time points after the last dosing

Cells were fixed with 4% PFA for 30 minutes, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat or donkey serum

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