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Furthermore, this neuropeptide can augment microglial expression of the pro-inflammatory enzyme cyclooxygenase-2 and its product prostaglandin E2, and elevate expression of the prostanoid receptors EP2 and EP4 to further potentiate inflammation (Rasley et al., 2004b)

Following administration in preclinical models: Intravenous administration provides immediate systemic availability Intramuscular injection demonstrates absorption with peak levels within 1-2 hours Individual peptide components show variable tissue distribution patterns Blood-brain barrier penetration demonstrated for certain neuropeptide fractions Distribution studies indicate that some peptide components can cross the blood-brain barrier, though the extent varies by molecular weight and specific peptide characteristics

Next we used the selected set of significant metabolites to build tissue-specific classifiers using an R wrapper for the rough-set ROSETTA toolkit 32,54