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The level of the pyruvate kinase muscle form (PKM2) of HepG2 cell Through with methanol incubation 30 minutes, then with 2 times (each 10 minutes) of TBS-T washing, use strip buffer subsequently in 50 of following incubations 30 minutes, peel off the lactic acid dehydrogenase trace with 100ml or more TBS-T washing 2 times (each 30 minutes) then.2 traces of scanning are to check out fully stripped in laser scanner.With methanol activation trace 5 seconds, with water washing 5 minutes, then with TBST washing 15 minutes.At room temperature, carried out 1 hour with the closed reagent among 5% TBS-T sealing trace, subsequently with TBS-T washing 3 times (1 time, about 15 minutes

It can eventually lead to serious complications if left untreated.