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Immunostaining, imaging, and immunofluorescence quantification For immunostaining, cells grown on coverslips were fixed by 4% PFA in PBSc/m (PBS plus 0.2 mM Ca 2+ and 2 mM Mg 2+ ), quenched (50 mM NH 4 Cl, 10 min), blocked (0.5% BSA, 0.01% Triton X-100, and DAPI in PBSc/m for 30 min), and incubated with primary antibodies for 1 h at room temperature in blocking buffer

Oxidative stress, aging, antioxidant supplementation and their impact on human health: an overview

Brightening happens gradually over multiple visits, and long-term maintenance may be recommended based on your lifestyle and sun exposure