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After separation by gel (10% SDS-PAGE) electrophoresis, the proteins were electrotransferred to PVDF membranes, blocked with 5% skim milk for 1 h, and incubated with antibodies against -tubulin (1:1000, Cell Signaling), MRP2 (1:1000, Cell Signaling), HIF-1 (1:1000, Cell Signaling), MMP9 (1:1000, Cell Signaling) and RKIP (1:1000, Cell Signaling) overnight at 4

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This work was supported by the National Natural Science Foundation of China [82205043], Natural Science Foundation of Sichuan Province [2024NSFSC1865 and 2025ZNSFSC1851], Sichuan Provincial Cadre Health Care Research Project [2024-503], Xinglin Scholars Discipline Talent Research Enhancement Program Postdoctoral Special Project [QJRC2023011 and BSH2023011], and Scientific Research Project of Health Commission of Chengdu (WXLH202403114 and WXLH202402022)

Bonvicini M, Travaglini S, Lelli D, Antonelli Incalzi R, Pedone C (2023) Is citicoline effective in preventing and slowing down dementia?a systematic review and a meta-analysis