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10.1093/toxsci/kfx230 37 WeiM.GuX.LiH.ZhengZ.QiuZ.ShengY.et al (2023)

TGP-free tt SI was then released from the column by His-tag-free 3C protease digestion, concentrated using a 100-kDa cutoff filtration membrane, heated at various temperatures, and used for FSEC-TS assay with the same setup as for GFP fusion protein except that the excitation and emission wavelength pair was changed to 280/350 nm