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Description

history of malabsorption or GI tract disorders or GI surgeries (i.e

In: Abbas VKAK, Fausto N, eds

Following a 2-min reaction at 4 C, 10 L of the mixture was injected onto a C-18 column (Agilent Purospher RP-18, 5 m, 4 250 mm) and the derivatized products were eluted at a flow rate of 1 mL/min at 37 C using a gradient elution with buffers A (80% 0.1 M sodium acetate pH 4.75 and 20% methanol) and B (20% 0.1 M sodium acetate pH 4.75 and 80% methanol) as described previously 7