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The estimation of total cellular GSH and GSSG levels was performed through GSH/GSSG-Glo Assay Kit (Promega) as per manufacturers instructions

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in the absence of ascorbic acid, the pro-oxidative activity is dominant, and these two effects are enhanced when it forms a complex with iron ( Although it is unclear whether myricetin can be used as an iron chelator, these results suggest that myricetin may play a key role in regulating iron homeostasis