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Gibco) supplemented with 2% FBS and incubated with rabbit anti-DNP antibody (1:100 dilution

Although defective tight junctions potentially lead to compromised intestinal barrier and the development and progression of gastrointestinal (GI) diseases, no FDA-approved therapies that recover the epithelial tight junction barrier are currently available in clinical practice

Available online at: 202 FarhabMAzizMWShaukatACaoMXHouZHuangSYet al

Further to this, GSTP1 stably expressed in MCF7 (Michigan Cancer Foundation 7 human breast adenocarcinoma) cells was found to associate with the plasma membrane-enriched fraction almost as strongly as the integral membrane protein Na + /K + -ATPase [24]