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Supporting this, we also found that homocysteine was mildly positively correlated with age (r+0.20, P ~0.06), implying that as thalassemic children grow olderand many undergo splenectomy homocysteine tends to rise.Nitrotyrosine levels were unexpectedly lower in the postsplenectomy group (mean, 15.99.1 nmol/L) compared to the presplenectomy group (21.49.8 nmol/L, P =0.035)

Both chemicals were dissolved in phosphate-buffered saline (PBS)

10.1182/blood.v96.7.2606 59 PorterJ.CappelliniM

The latter modification is consistent with our recent report that parkin thiols participate in redox chemistry to neutralize highly reactive molecules , e.g., H 2 O 2 and dopamine metabolites [53], as well as with the literature on transient S- glutathionylation of redox-sensitive proteins as a cytoprotective mechanism against rising concentrations of ROS [27]