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The erythroblasts were treated with hemin (75 M), the oxidized form of heme, under hypoxic condition (1% O 2 ), with or without 50 M DMF in a two-phase culture system 31 (Supplementary Fig

Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s

Importantly, patterns of age-related muscle loss appear to be different between sexes, with men demonstrating a greater rate of relative loss in muscle mass with age and females having a greater prevalence for sarcopenialikely due to males having a higher muscular reserve compared to females (de Jong et al., 2023)

A 2018 study showed that increased glutathione in the body enhanced immune function and reduced markers of oxidative stress