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Embryonic cochleae with the brain hemisected were fixed overnight at 4C in 4% PFA in 1x PBS

Exp Neurol 128(1):103114 Kidd PM (2008) Alzheimers disease, amnestic mild cognitive impairment, and age-associated memory impairment: current understanding and progress toward integrative prevention

A sample size calculation using a two-tailed t-test to achieve a power of 80% and alpha of 5%, with a SD = 0.8 and expected difference of 1.2 between groups, indicates that 9 women per study group would be required for the present study