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From a manufacturing standpoint, although genetic fusion utilizes a simplified single-expression system, fusion constructs frequently require extensive, antigen-specific optimization because large or structurally complex antigens often express as insoluble aggregates or inclusion bodies, as exemplified by HBc fusions with SARS-CoV-2 RBD in which only low yields of soluble VLP were obtained without scaffold modification (Petrovskis et al., 2024)

Albrecht ED, Babischkin JS, Lidor Y, Anderson LD, Udoff LC, Pepe GJ

As shown in Figures 2A, B , cDCs from DEHP-exposed mice significantly increased the percentage of IL-4 + , but not IFN- + CD4 + T cells