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Errors here cascade through your entire protocol, causing incorrect concentrations, degraded peptides, and unreliable results

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For each recording (15 laser stimulations), the cerebral blood flow (CBV) was normalized into a relative steady-state value (rCBV) and calculated as the following: rCBV = (CBV(t) - CBV 0 )/CBV 0 , where CBV(t) is the power doppler value t seconds after the start of laser sequence, and CBV 0 is the baseline in the ROI