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2 HMC3 cell line The HMC3 cell line was originally established in 1995 through the SV40-dependent immortalization of primary microglial cultures derived from 812-week-old human embryonic spinal cord and cortical tissues (164), primary human microglia were isolated by circular shaking from mixed neural cultures and exhibited slow proliferation kinetics, reaching confluence within 812 days

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Bioavailability therefore depends on both chemical stability and transcellular passage