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Ogata Y, Goda S, Toyama T et al (2013) The 4-tert-butylphenol-utilizing bacterium sphingobium fuliginis OMI can degrade bisphenols via phenolic ring hydroxylation and meta-cleavage pathway

For example, female mice with IGF-1R knock-out were shown to be completely sterile, with no antral follicles and a 90% reduction in serum E2 levels (66)

Briefly, independent cultures of Agrobacterium carrying pTRV1 or pTRV2-based constructs were resuspended in 10 mM MgCl 2 , 10 mM MES (pH 5.6) and 150 M acetosyringone at OD 600 = 0.8, and incubated for 2 h at 28 C in the dark