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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

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Injectable GHK-Cu achieves higher systemic and tissue concentrations and is the standard for clinical protocols targeting wound healing, post-procedure recovery, and longevity applications