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In other preferred embodiments of of the linker of Formula (IV), Z is independently selected from the group consisting of one or more amino acid(s), one or more N- substituted amino acid, optionally substituted polyether, C1-C12 alkylene, arylene having 6 to 10 ring atoms, C3-C8 cycloalkylene, heterocycloalkylene having 5 to 10 ring atoms, heteroarylene having 5 to 10 ring atoms, C2-C10 alkenylene, and any combination thereof, said alkylene and alkenylene being optionally interrupted by one or more heteroatom or chemical groups selected from -O-, -S-, -C(O)-, -NR-, -C(O)NR-, -NR-C(O)-, -NR- C(O)-NR'-, -NR-C(O)-O-, -O-C(O)NR- and triazole, and said alkylene, arylene, cycloalkylene, heterocycloalkylene, heteroarylene, and alkenylene being optionally substituted with one or more of the substituents selected from : halogen, oxo, -OH, -NO2, -CN, C-i-Ce alkyl, C3-C6 cycloalkyl, heterocyclyl having 5 to 10 ring atoms, aryl having 6 to 10 ring atoms, heteroaryl having 5 to 10 ring atoms, Ci-Ce alkoxy, Ci-Ce haloalkyl, Ci-Ce haloalkoxy, -(CO)-R, -O-(CO)-R, -(CO)-O-R, - (CO)-NRR, -NR-(CO)-R, and -NRR

coli (Zymo) and plated O/N at 37 C on 2xTYAG agar

IDY:= Inactivated Dry Yeasts

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