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In animals without tau, he adds, amyloid is not terribly toxic

In mammalian cells, -glutamylcysteine synthetase is effectively inhibited by a GSH feedback, whereas glutathione synthetase is not subject to the negative feedback 9,30

If the interacting protein that youre analyzing also binds to the GST only control, then this may be through non-specific interactions to the beads or to GST (Figure 8)