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ChIP-qPCR assays performed using an anti GFP antibody in HEK293T cells transduced with GFP-ASXL1 1-645 -WT or GFP-ASXL1 1-645 -I574D as well as with GFP-ASXL1 1-591 -WT or GFP-ASXL1 1-591 -I574D showed that both truncated variants of ASXL1 localize to the promoters of ECH1 , POGLUT1 and PGK1 independently of binding of BRD4 (Fig