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Following affinity chromatography, a nucleic-acid-free AGO fraction (as judged by 260/280 ratio) was isolated by cation-exchange chromatography (Source 15S or MonoS, Cytiva) with a gradient of 0500 mM KCl in 20 mM Tris, pH 8.0

Brain, Behavior, and Immunity 87:715724 Dehhaghi M, Heng B, Guillemin GJ (2023) The kynurenine pathway in traumatic brain injuries and concussion

Agar gel with a 1.5%weightvolume ratio is used as a base for the films, which are subsequently covered with pH paper to measure the surface pH

The circadian clock is entrained by light perceived by phytochromes (red and far-red [FR] light receptors), cryptochromes (blue light receptors), and temperature (Greenham and McClung, Arabidopsis thaliana (McClung, 2006