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doi: 10.1007/s00726-017-2429-y

Then the COCs were washed three times in DPBS, twice in the maturation medium (the medium was TCM-199 supplemented with 10% FBS, both FBS and TCM-199 were purchased from Thermo Fisher Scientific), and cultured in an incubator with a CO 2 concentration of 5% at 38.5C for 27 h