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Tetrahydrobiopterin improves endothelium-dependent vasodilation in chronic smokers: evidence for a dysfunctional nitric oxide synthase

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For comparisons between 2 groups, 2-tailed Students t test for unpaired or paired data was used

Oocytes were washed four times with DPBS and incubated in staining working solution containing 3 M Nile Red (MCE, Shanghai, China) in the dark for 30 min at room temperature