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6 Limitation and Future perspective Although current research has revealed the critical role of ferroptosis in the pathological progression of AD, several limitations remain to be addressed in this field: (1) Existing evidence primarily relies on animal models or in vitro cellular experiments, failing to comprehensively reflect the spatiotemporal dynamics of iron metabolism dysregulation in the brains of human AD patients

[22] J.B

We attempted to characterize these interactions by SPR but detected very low binding responses to perlecan with concentrations up to 200 nM (data not shown), an observation which may be related to the low overall perlecan affinity

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