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This dysregulated cholesterol metabolism affects immune cell function, inducing mitochondrial dysfunction in CD8 + T cells and MDSCs ( BOS complex subunit transmembrane protein 147 (TMEM147) is upregulated in hepatocellular carcinoma (HCC) cells

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Cells were subsequently incubated with an Alexa Fluor-conjugated secondary antibody (1:1000, A-11012, Thermo Fisher Scientific) for 30 minutes at room temperature, protected from light

452,455 Inhibition of the ETC leads to aspartate depletion, triggering ATF4 upregulation and increasing the transcription of genes related to amino acid transport and synthesis