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The beads were then washed with RIPA lysis buffer 3 times, and bound proteins were eluted by boiling in SDS-PAGE loading buffer

Oncol Rep 29:13221332 Bi F, Wang J, Zheng X, Xiao J, Zhi C, Gu J, Zhang Y, Li J, Miao Z, Wang Y (2021) HSP60 participates in the anti-glioma effects of curcumin