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Western blot was used to detect the expression of osteogenic transformation marker proteins, azo coupling was used to detect alkaline phosphatase activity, and alizarin red S staining was used to detect the amount of calcium deposition

For example, naringin (naringenin-7- O -rhamnoglucoside) as a flavanone member, if subjected to sugar hydrolysis yields naringenin as the aglycone part (Fig