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Proteins were transferred to PVDF membranes (Thermofisher) using a Power BlotterSemi-dry Transfer System (Thermofisher) and incubated overnight with SHMT2 (E7F4Q) Rabbit mAb (Cell signaling, #3344) and vinculin Mouse mAb (Sigma-aldrich, #V9131) antibodies diluted in 5% skimmed milk in Tris-Buffered NaCl Solution with Tween 20 (TBST)

Researchers commonly investigate: tissue repair pathways

In sum, genetic perturbations of IF genes ifd-1 and ifd-2 , and aggresome adaptor genes ftt-2 and hsp-1 suppress exopher levels, demonstrating a potential link between these aggresome components and exopher formation