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The present study will investigate the reversal of long-term glutamine blockade-induced upregulation of PD-L1 in bladder cancer cells through combination therapy with JHU083 and gefitinib [105]

Plates were incubated at 37C for 2 h and then fluorescence was measured with excitation at 360 nm and emission at 460 nm using a ClarioStar Monochromator Microplate Reader (BMG LABTECH, Ortenberg, Germany)

10.1002/jcsm.12857 87 FarooquiA

However, it is difficult to determine if oxidative damage represents the primary cause or a secondary consequence of this disease [56, 57] and whether oxidative damage appears early or late in the course of the disease