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For practical purposes, the stable gastric pentadecapeptide BPC 157 was given daily, intraperitoneally or perorally, in drinking water, using the previous efficacious regimens as in the other external fistulas healing trials (10-12)

The crude was further diluted with water (9 mL) and purified by HPLC (eluent A 0.1% TFA in water, eluent B 0.1% TFA in acetonitrile/water 4/1 v/v, 50 C, detection at 215 nm, 6 mL min 1 , 020% eluent B in 5 min, then 2045% eluent B in 60 min, C18XBridge column) to give the cyclic peptide cK1-1 as a white solid after lyophilization (1.71 mg, 31%)

Archimedes wanted to melt the gold material into a known volume and weigh it, thus determining the density