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Copper overload Copper overload disrupts the expression and function of antioxidant enzymes and induces oxidative stress, while oxidative stress and increased ROS levels are thought to be the main cause of Cu-induced cytotoxicity (57,58)

Hydrochlorothiazide may result in hypokalemia and/or hypomagnesemia and increase the risk of drug-induced proarrhythmias (including torsade de pointes) for drugs such as arsenic trioxide, bepridil, cardiac glycosides, levomethadyl, mibefradil, and sotalol

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The colored end-product of the assay demonstrated remarkable stability at room temperature, with the absorbance at 412 nm remaining stable for over three hours