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Additionally, FOXO4-DRI reduced the DHE-positive cell count, indicating decreased ROS levels under OGD conditions (Figure 4F)

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Samples (20 l) were mixed with 5 l of 5x loading buffer (0.3 M Tris-HCl pH = 6.8, 25% -mercaptoethanol, 50% glycerol, 10% SDS, 1% bromophenol blue), heated to 95 C for 5 min, and 15 l of samples were applied to the gel together with 5 l of Thermo Scientific PageRuler Unstained Broad Range Protein Ladder

however, it needs building blocks for repair either from supplementation or diet