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B12-deficient breeders Edema Hemorrhages Beak shortening Myotrophy of legs and perosis Fattiness of the liver, heart, and kidneys Fewer myelinated fibers in the spinal cord high incidence of embryonic death at 17 days of incubation Although several concerns can be seen in vitamin B12-deficient chickens, the occurrence can still be considered highly unlikely or rare if they are regularly fed a complete diet or grown on litter where animal-based ingredients are used

Expression of C-terminal His-tagged human GCLC was induced with 1 mM IPTG at 16C for 16 h in Escherichia coli

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