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The injection can be performed by a physician, or by a nurse or assistant under the [direct supervision] of the physician.

This procedure is not expected to evaluate the implication of melatonin or the pineal gland in the mechanism of action of the antidepressants, but rather to produce a proxy of the immediate events that follow the consequences of antidepressants at synaptic level

Neuronal hyperactivity disturbs ATP microgradients, impairs microglial motility, and reduces phagocytic receptor expression triggering apoptosis/microglial phagocytosis uncoupling

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