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Eluent A consisted of acetonitrile:water (50:50, v/v, both ULC/MS-CC/SFC grade, Biosolve B.V.) and Eluent B of 2-propanol:acetonitrile:water (85:10:5, v/v/v), both containing 5 mM ammonium formate (MS grade, Sigma Aldrich) and 0.1% (v/v) formic acid (ULC/MS-CC/SFC grade, Biosolve B.V.)